Full Name: Locus of enterocyte effacement
Strain: O157:H7 EDL933
Location: 4649752~4692545 bp
Size: 43 Kbp
G+C content (%): 40.91/50.8 (PAI/Genome)
Similar to the EPEC LEE, this PAI is involved in the production of proteins associated with intimate adherence to intestinal epithelial cells, initiation of host signal transduction pathways, and the formation of A/E lesions.
The EHEC LEE comprises 43,359bp in comparison to 35,624bp for EPEC LEE, and contains 41 genes are common to both the EHEC and EPEC LEEs. The ORFs that are not present in the EPEC LEE fall within a putative prophage, designated 933L, that is located next to the selC locus. The 933L prophage is a member of the P4 family of cryptic prophages.
A few genes in EPEC and EHEC LEEs show markedly higher variablility: espB (25.99% difference), espD (19.64%), and espA (15.37%), eae (12.77%) and the intimin receptor gene, tir (33.52%).
Elliott SJ, et al., 2000. The locus of enterocyte effacement (LEE)-encoded regulator controls expression of both LEE- and non-LEE-encoded virulence factors in enteropathogenic and enterohemorrhagic Escherichia coli. Infect. Immun. 68(11):6115-6126.
Iyoda S, et al., 2006. The GrlR-GrlA regulatory system coordinately controls the expression of flagellar and LEE-encoded type III protein secretion systems in enterohemorrhagic Escherichia coli. J Bacteriol 188(16):5682-92.
Naylor SW, et al., 2005. Escherichia coli O157 : H7 forms attaching and effacing lesions at the terminal rectum of cattle and colonization requires the LEE4 operon. Microbiology 151(Pt 8):2773-81.
Schmidt MA, 2010. LEEways: tales of EPEC, ATEC and EHEC. Cell Microbiol 12(11):1544-52.
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