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Pyocyanin |
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One of the secreted pigments that give P.aeruginosa laboratory cultures their characteristic blue-green color. It is a redox-cycling compound consisting of modified phenazine ring that is derived from anthrinilate. The precursor molecule of pyocyanin is chorismic acid, the end product on the shikimate pathway. The conversion of chorismic acid to phenazine-1-carboxylic acid (PCA) is controlled by seven genes, encoded by two operons (phzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2). PCA is converted to 5-methylphenazine-1-carboxylic acid betaine (MPCBA), by means of a phenazine-specific methyltransferase (PhzM). In the second step, MPCBA is catalysed by flavin-dependent monooxygenase (PhzS), involving the hydroxylation of the MPCBA betaine to 1-hydroxy-5-methyl phenazine, i.e., pyocyanin. ... |
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AnsP |
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Belongs to the Phagosomal transporter (Pht) subclass of major facilitator superfamily that is exclusively found among intracellular pathogenic bacteria. ... |
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ArgP |
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ArgP is the closest paralogue of Francisella GadC within the APC (amino acid-polyamine-organocation) family of transporter. ... |
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Biotin synthesis |
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Biotin is an important enzyme cofactor that acts as a covalently bound prosthetic group. Biotin plays critical roles in central metabolic processes involving carboxylation, decarboxylation and transcarboxylation. F. novicida has evolved two distinct biotin protein ligases BplA and BirA with specific roles. Only BplA is required for pathogenicity. ... |
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GadC |
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Glutamate permease GadC helps the bacterium to resist the oxidative stress generated by the NADPH oxidase. This transporter is critically required for proper bacterial phagosomal escape. ... |
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GGT (γ-glutamyl transpeptidase) |
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Cleavage of cysteine containing peptides (glutathione and γ-glutamyl-cysteine peptides) by GGT activity thus provides the essential source of cysteine required for intracellular multiplication. ... |
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IleP |
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Belongs to the Phagosomal transporter (Pht) subclass of major facilitator superfamily that is exclusively found among intracellular pathogenic bacteria. ... |
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Purine biosynthesis |
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The purMCD locus encodes three enzymes (phosphoribosylaminoimidazol synthetase, SAICAR synthetase, and phosphoribosylamine-glycine ligase) required for the de novo synthesis of purine nucleotides. ... |
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Pyrimidine biosynthesis |
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CarB, CarA, and PyrB encode the large and small subunits of carbamoylphosphate synthetase and aspartate carbamoyl transferase, respectively. These enzymes catalyze the first two steps in the pyrimidine nucleotide biosynthetic pathway in many bacteria, including Francisella, and are required for the virulence of several pathogens, including Salmonella and E. coli. ... |
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Pht (Phagosomal transporter) |
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Enables transmissive L. pneumophila to assess their phagosomal nutrient supply before reentering the cell cycle. ... |
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